Thermoluminescence
Probe of PSII Photochemistry
About...

Thermoluminescence instruments are designed to investigate structure of energetic levels in the Photosystem II. Light-induced charge separation in the Photosystem II reaction centers results in accumulation of radical pairs that store the absorbed light energy. Heating induces recombination of these radical pairs and it triggers light emission and formation of characteristic thermoluminescence glow curves.

Key Features

  • Highly sensitive photomultiplier detector
  • Precisely linear heating process controlled by thermo-electric cooler regulation in the temperature range from -100 to 200°C
  • Air input port for defined atmosphere inside the measuring chamber


  • Optically isolated design allows measurements under ambient laboratory conditions
  • Touch-screen display for real time data visualization and basic device settings
  • User-friendly software interface for automated protocols, scripting, data visualization and manipulation

Thermoluminescence


PSI Thermoluminescence instruments are designed to investigate structure of energetic levels in the Photosystem II. Light-induced charge separation in the Photosystem II reaction centers results in accumulation of radical pairs that store the absorbed light energy. Heating induces recombination of these radical pairs and it triggers light emission and formation of characteristic thermoluminescence glow curves. The shape and the peak position temperature of the different thermoluminescence bands provide valuable information about the energetic stability of the respective radical pairs as well as about the functioning of the Photosystem II reaction centers. Interpretation of the obtained data requires a through understanding of the charge pairs responsible for generating different thermoluminescence bands. High-temperature thermoluminescence appears as a result of accumulation of lipid peroxides and can be used as a simple and efficient tool to monitor oxidative stress in plants.



Samples


  • Algae and cyanobacteria suspensions
  • Plants leaves
  • PS II preparations
  • Thylakoids
  • Lichens
  • Photosynthetic bacteria




Applications

Photosynthetic TL Bands


  • Investigation of PSII functions:
  • - Function of O2 evolving complex
    - Charge storage
    - S state oscillations
    - S state stability and deactivation

  • Redox and protonation states of PSII electron acceptors
  • Perturbations in PSII induced by stress factors - TL stress indices
  • Adaptation (thermophilic, cold tolerance)
  • Resistance and susceptibility to herbicides
  • Characterization of PSII mutants

High Temperature TL Bands


  • Detection of oxidative stress in plants and algae
  • Investigate lipid peroxidation level



Software


  • Creation and saving of experimental protocols
  • FluorWin Wizard for comfortable protocols scripting
  • Retrieval and export of experimental data
  • Data manipulation and visualization
  • Windows 7, or higher compatible



Versions



Thermoluminescence TL 6000/ST


  • Temperature range from -25 to 70 °C
  • Maximal linear heating rate 1.5 °C/s
  • Temperature regulation using Peltier element and water cooling unit
  • Apropriate for investigation of Q, A, B1, B2, C, AG glow curve peaks



Thermoluminescence TL 6000/ET


  • Temperature range from -100 to 200 °C
  • Maximal linear heating rate 1.8 °C/s
  • Temperature regulation using nitrogen tank and resistance heater
  • Apropriate for investigation of Z1, Q, A, B1, B2, C, AG and lipids glow curve peaks



Pricing


Device
Version Characteristics Price
Thermoluminescence System TL 6000/ST Standard version providing temperature control in the range of -25°C to +70°C. Enquire for price,- €
Thermoluminescence System TL 6000/ET Extended temperature version providing temperature control in the
range of -100°C to +200°C
Enquire for price,- €