Thermoluminescence
Quick, reliable and easy measurements in the field or laboratory
About...

Thermoluminescence instruments are designed to investigate structure of energetic levels in the Photosystem II. Light-induced charge separation in the Photosystem II reaction centers results in accumulation of radical pairs that store the absorbed light energy. Heating induces recombination of these radical pairs and it triggers light emission and formation of characteristic thermoluminescence glow curves.

Key Features

  • Different versions for measuring in broad temperature range from -100 to +200 °C
  • Variable high-intensity light source (typically 627 nm) used as a single turnover light for S state transition analysis
  • Variable long-duration actinic light source (typically 627 nm) for illumination at predefined temperatures
  • Sensitive photomultiplier for thermoluminescence glow peak detection
  • Protocol controlled electronic shutter preventing the photomultiplier from damage


  • Precisely linear heating process controlled by thermo-electric cooler regulation in the whole heating range
  • Ambient light protection enabling measurements in standard laboratory conditions
  • Precisely linear heating process controlled by thermoelectric cooler regulation in whole heating range
  • Ambient light shielding enables measurements at standard laboratory conditions

Thermoluminescence


PSI Thermoluminescence instruments are designed to investigate structure of energetic levels in the Photosystem II. Light-induced charge separation in the Photosystem II reaction centers results in accumulation of radical pairs that store the absorbed light energy. Heating induces recombination of these radical pairs and it triggers light emission and formation of characteristic thermoluminescence glow curves. The shape and the peak position temperature of the different thermoluminescence bands provide valuable information about the energetic stability of the respective radical pairs as well as about the functioning of the Photosystem II reaction centers. Interpretation of the obtained data requires a through understanding of the charge pairs responsible for generating different thermoluminescence bands. High-temperature thermoluminescence appears as a result of accumulation of lipid peroxides and can be used as a simple and efficient tool to monitor oxidative stress in plants.



Samples


  • Typical samples are leaf segments, algal cells, thylakoids, or PSII preparations
  • Sample disc made of gold-plated copper has a diameter of 14 mm (in standard version)
  • Sample disc made of gold-plated copper for the extended temperature version has a diameter of 22 mm




Applications


  • Non-invasive monitoring of the PSII electron transport
  • PSII adaptation and responses to abiotic and biotic stresses, structural modifications, mutations
  • Tests of herbicides cos TL allows to probe energetic stability of reduced quinone electron acceptors
  • Redox reactions of PSII in thylakoid membrane
  • Interpreting changes in the redox potentials of donors and acceptors on the basis of a shift in glow peak temperature
  • Oscillation pattern of TL can denote the ‘‘S’’ state transition and can be used for a titre of the Mn clusture of oxygen evolving complex



Software


  • Creation and saving of experimental protocols
  • FluorWin Wizard for comfortable protocols scripting
  • Retrieval and export of experimental data
  • Data manipulation and visualization
  • Windows 7, or higher compatible



Versions



Thermoluminescence TL 6000/ST


  • standard version intended for investigations of Q, A, B1, B2, C, and AG glow curve peaks





Thermoluminescence TL 6000/ET


  • extended temperature version for temperature range from -100 °C to +200 °C can additionally monitor Z1 glow curve peak (liquid nitrogen tank included in the price)


Specifications


Temperature Range:
Standard version: -25 to +70 °C
Liquid Nitrogen version: -90 to +70 °C.
High-Temperature version: -15 to +170 °C

Temperature Mode: Linear temperature change:
Thermoluminescence 0.1 – 2 °C/s
Chemoluminescence 0.1 – 1 °C/s

Overheating Protection:
Yes

Minimum Sampling Period:
100 ms

Temperature Control:
Manual (constant temperature) or Autonomous-protocol defined temperature profiles.

Sample Disc:
made of gold-plated copper standard and low temperature version: diameter 14 mm, high temperature version: diameter 22 mm

Typical Sample:
Algal or cyanobacterial suspensions; Leaf segments

Saturating Pulse Illumination:
625 nm, up to 250,000 µmol.m-2.s-1

Actinic Illumination:
625 nm, up to 1,500 µmol.m-2.s-1

Sensor:
Photomultiplier with software control of the sensitivity

Spectral Response:
300 – 900 nm

Switch-On Delay:
100 ms

Ambient Light Protection:
Yes

Control:
Custom defined protocols with variable timing, special language and scripts • Communication: RS232/USBc

Software:
FluorWin 3.7

Electrical:
90 V – 240 V

Pricing


Device
Version Characteristics Price
Thermoluminescence System TL 6000/ST Standard version providing temperature control in the range of -25°C to +70°C. Enquire for price,- €
Thermoluminescence System TL 6000/ET Extended temperature version providing temperature control in the
range of -100°C to +200°C
Enquire for price,- €